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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Vector Laboratories succinylated vector laboratories cat
Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures <t>of</t> <t>immunohistochemical</t> staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine <t>(DAB)</t> was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
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Image Search Results


Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures of immunohistochemical staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine (DAB) was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).

Journal: Neuro-Oncology

Article Title: Systemic AAV9-IFNβ gene delivery treats highly invasive glioblastoma

doi: 10.1093/neuonc/now097

Figure Lengend Snippet: Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures of immunohistochemical staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine (DAB) was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).

Article Snippet: VECTASTAIN Elite ABC Kit (PK-6100, Vector Laboratories) and DAB substrate kit (SK-4100, Vector Laboratories) were used for immunohistochemical detection and Mayer′s hematoxylin (Sigma) as counterstain.

Techniques: Plasmid Preparation, Immunohistochemical staining, Staining, Expressing, Injection