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Image Search Results
Journal: Neuro-Oncology
Article Title: Systemic AAV9-IFNβ gene delivery treats highly invasive glioblastoma
doi: 10.1093/neuonc/now097
Figure Lengend Snippet: Astrocytes and vascular endothelial cells are the predominant brain cells transduced upon vascular delivery of scAAV9-EGFP vector in glioblastoma (GBM) tumor-bearing mice (A) Representative pictures of immunohistochemical staining for EGFP-expressing cells in brain sections from GBM8-Fluc-bearing mice 3 weeks after tail vein delivery of 3 × 1011 gc/mouse of scAAV9/CB-EGFP and scAAV9/TBG-EGFP. Control mice were infused with PBS. 3,3′-diaminobenzidine (DAB) was used for detection of EGFP-positive cells (brown) in the brain and hematoxylin was used for counterstaining. (B) High magnification pictures of 4 different brain areas (identified in A by boxed small case letters) from mice injected with scAAV9/CB-EGFP vector. Large arrows in Ba and Bc indicate EGFP-expressing cells with astrocyte morphology. Small arrows in Bb and Bd indicate EGFP-positive cells with vasculature morphology. Images from one animal representative of each group (n = 3).
Article Snippet: VECTASTAIN Elite ABC Kit (PK-6100, Vector Laboratories) and
Techniques: Plasmid Preparation, Immunohistochemical staining, Staining, Expressing, Injection